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β-(1,4)-GalNAc transferase catalyzes the transfer of glycosyl groups to acceptor sugars such as N-acetylglucosamine, forming a β(1→4) linkage. This enzyme exhibits broad substrate tolerance and can recognize a variety of glycosyl groups, including 6-azidoGalNAc. It plays important roles in diverse biological processes such as embryonic development, immune and inflammatory responses, and tumorigenesis and tumor progression.
Currently, β-(1,4)-GalNAc transferase is one of the key tools for enzymatic site-specific conjugation in antibody-drug conjugate (ADC) development.
In addition, accurate quantification of residual enzyme levels is critical to ensuring product quality, safety, and regulatory compliance.
For research and in-process quality control use only. Not for diagnostic use in humans or animals.
Product images are for reference only.
Applications
This product adopts a sandwich ELISA format to quantify β-(1,4)-GalNAc transferase in test samples.
Advantages
(1) Rapid 3-hour detection with high precision and reproducible data.
Simple operation: pre-coated plates with streamlined procedures, eliminating complex sample handling.
No large-scale equipment required: assay can be completed on a standard microplate reader, with results available in 3 hours.
(2) Dual advantages in sensitivity and linear range
Limit of detection (LOD): 50 pg/mL, enabling reliable quantification of trace residues.
Linear range: 50–5000 pg/mL, supporting accurate quantification of both low- and high-concentration samples without dilution.
(3) Maximized anti-interference performance and stable batch-to-batch performance
Spike recovery: 85%–115%. Multiple interfering proteins have been verified to have no significant effect on assay results.
Inter-batch coefficient of variation (CV): ≤10.0%, ensuring lot-to-lot consistency through strict quality control.
Information
(1) Product Name：β-(1,4)-GalNAc transferase Detection ELISA Kit
(2) Catalog No. : K068-1
(3) Size : 48T / 96T
(4) Sensitivity
Limit of Detection (LOD): 50 pg/mL
Limit of Quantitation (LOQ): 100 pg/m
(5) Linear range
The linear range is from 50 to 5000 pg/mL.
(6) Recovery rate
The spike recovery ranges from 85.0% to 115.0%.
(7) Inter-batch coefficient of variation
The inter-batch coefficient of variation (CV) is ≤ 10.0%.
(8) Specificity
This kit can detect both native and recombinant β-(1,4)-GalNAc transferase.