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Recombinant IgdE is recombinantly expressed in Escherichia coli. It specifically recognizes human IgG1 and cleaves it above the hinge region into Fab and Fc fragments. The cleavage site on human IgG1 is KSCDKT/HTCPPC.
It is active over pH 6–8, with an optimal pH of 6.5–7.5, and does not require cofactors such as reducing agents.
Product images are for reference only.
Applications
（1）Subunit LC/MS analysis.
（2）Pairwise glycan analysis.
（3）Characterization of bispecific or multispecific antibodies.
（4）Affinity screening.
（5）Higher-order structure studies.
（6）Research on hinge-mutated antibodies.
Advantages
（1）Cleaves above the hinge region, effectively avoiding interference from hinge region mutations.
（2）No requirement for cofactors such as reducing agents for enzymatic digestion.
Information
（1）Product Name: Recombinant IgdE, Lyophilized
（2）Catalog No. : YJ-O-218
（3）Size: 1 KU / 2 KU
（4）Storage temperature: -20 °C