This kit contains O-Glycosidase and α2-3,6,8 Neuraminidase.

O-Glycosidase is also known as endo-α-N-acetylgalactosaminidase. After removal of terminal sialic acid residues from glycoproteins with sialidase, O-Glycosidase catalyzes the removal of O-linked disaccharides with core 1 and core 3 structures from glycoproteins. This product is glycerol-free in storage buffer, ensuring excellent compatibility with downstream mass spectrometry and HPLC. It contains a His-tag for convenient removal via chromatography.

α2-3,6,8 Neuraminidase hydrolyzes sialic acids linked via α2-3, α2-6, and α2-8 glycosidic bonds on glycoproteins and oligosaccharides. The hydrolysis rate for α2-3 linkages is higher than that for α2-6 and α2-8 linkages, while the rates for α2-6 and α2-8 linkages are similar.