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Vaccinia Capping Enzyme is expressed in Escherichia coli and consists of two subunits, D1 and D12. It possesses RNA triphosphatase, guanylyltransferase and guanine methyltransferase activities, enabling the attachment of a 7-methylguanosine cap structure (m7Gppp) to the 5′-end of RNA (m7Gppp5′N). This cap structure enhances mRNA stability, transport and translation efficiency, and is an essential modification for in vitro mRNA synthesis.
Product images are for reference only.
Applications
(1) In vivo / in vitro 5′-end capping of mRNA(2) GTP labeling of the 5′-end of mRNA
Advantages
(1) Completes RNA capping within one hour with capping efficiency up to 100%
(2) Free from exogenous DNase and RNase contamination
Information
(1) Product Name: Vaccinia Capping Enzyme
(2) Catalog No. : YJ-O-032
(3) Size: 125 U / 500 U / 1 KU
(4) Storage temperature: -20 ℃