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T7 RNA Polymerase V2 is expressed in Escherichia coli, with its sequence derived from a mutant of T7 bacteriophage RNA polymerase gene. It features high synthesis efficiency and low double-stranded RNA (dsRNA) byproduct formation. The enzyme is highly specific: in the presence of magnesium ions, it exclusively uses single- or double-stranded DNA containing the T7 promoter sequence (5’-TAATACGACTCACTATAG*-3’) as a template, and employs NTPs as substrates to synthesize RNA complementary to the DNA strand downstream of the promoter. Both linear blunt-ended and 5’-overhanging double-stranded DNA can serve as templates for T7 RNA Polymerase V2, making linearized plasmids and PCR products suitable templates for in vitro RNA synthesis.
Product images are for reference only.
Applications
(1) In vitro synthesis of conventional mRNA
(2) In vitro synthesis of mRNA labeled with radioisotopes, fluorescent dyes, biotin or digoxigenin
(3) Synthesis of RNA probes
Advantages
(1) Highly specific, recognizing only the T7 promoter sequence
(2) Superior synthesis activity compared with wild-type enzyme, with a lower dsRNA ratio
Information
(1) Product Name: T7 RNA polymerase V2
(2) Catalog No. : YJ-O-316
(3) Size: 1 KU / 5 KU / 10 KU / 50 KU
(4) Storage temperature: -20 ℃